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KMID : 0368419910340030215
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Journal of Plant Biology
1991 Volume.34 No. 3 p.215 ~ p.221
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Photosynthetic Characteristics of Polyvinylalcohol-Immobilized Spinach Chloropfasts
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Park, In Ho/朴仁虎
Seo, Sang Hee/Lee, Hae Joo/Lee, Chin Bum/Sunwoo, yang Il/徐尙希/李蕙珠/李鎭範/鮮宇洋一
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Abstract
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Photosynthetic properties of polyvinylalcohol (PVA)-immobilized chloroplast especially regarded to stability of photosynthetic electron transport and the fluorescence induction pattern were studied. When isolated spinach chloroplasts were immobilized with PVA, it showed good preservation of photosynthetic electron transport activity, especially PS Ⅱ activity, during storage at -15℃, 4℃ and 20℃. And immobilized chloroplasts revealed similar thermostability of whole chain electron transport to free chloroplasts. And the absorption peak of red band of chloroplasts showed the blue-shift of 2-4 ㎚ after immobilization. F_v/F_m ratio of chlorophyll fluorescence slightly decreased after immobilization. While light pulse after continuous light do not induced the additional fluorescence rise. This means chlorophyll fluorescence at room temperature reached to F_max under continuous light in the immobilized chloroplasts. It seems that PVA may be a good candidate for immobilization matrix for the preservation of photosynthetic function of thylakoids and for the continuous use of chloroplast membranes of higher plants for solar energy storage and conversion.
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